Nanocrystalline silver (NPI 32101) has been demonstrated to have antimicrobial and anti-inflammatory properties

The purpose of this study was to assess the effect of NPI 32101 in a rat model of ulcerative colitis and the possible mechanisms of action of the effects observed. NPI 32101, 4 mg/kg intracolonically or 40 mg/kg orally, significantly reduced colonic inflammation compared to the placebo and no-treatment groups. Sulfasalazine (100 mg/kg), either intracolonically or orally, also reduced colonic inflammation.

NPI 32101 significantly suppressed the expression of matrix metalloproteinase (MMP)-9, tumor necrosis factor (TNF)-α, interleukin (IL)-1β, and IL-12, whereas sulfasalazine suppressed MMP-9, IL-1β, and TNF-α, but not IL-12, compared to placebo. MMP-9 activity was reduced by NPI… 32101 and sulfasalazine.

NPI 32101 administered intracolonically or orally decreases ulcerative colitis in a rat model and is as effective as sulfasalazine. NPI 32101 treatment suppresses the expression and activity of MMP-9 and the expression of TNF-α, IL-1β, and IL-12, mechanisms by which NPI 32101 may exert its anti-inflammatory effects. NPI 32101 may have therapeutic potential for treatment of ulcerative colitis.

Mercury ions (Hg(2+)) are one of the most dangerous pollutants

Even at low concentration, it causes serious environmental and health problems. Current methods for the detection of Hg(2+) in environmental samples are tedious and time consuming because they require sophisticated instrumentation and complicated sample pre-treatment processes. In this work, a novel probe with high selectivity towards Hg(2+) was synthesized and a one step competitive immunochromatographic assay based on the probe for the detection of Hg(2+) was developed and applied for water samples.

The detection conjugate was immobilized on one end of the nitrocellulose membrane (detection line) and anti-BSA polyclonal antibody was immobilized on the other end of the membrane (control line). Hg(2+) in samples competed with… the probe to bind with immobilized detection conjugate.

The visual detection limit of Hg(2+) in spiked water samples was found to be about 1 ppb. The qualitative assay can be performed within 15 min. The advantages of the technique are rapidity, low cost and without the need of any equipment and complicated sample preparation.